In order to
explain the effects of these two hormones on cAMP levels in this cells I’m
going to describe each of the three cases on the graph.
In the
first column of the first case, in which only acts Nerve Growth Factor(NGF) the
cAMP levels are of 20 micromoles. When the A peptide is added to the NGF, the
amount of cAMP gets reduced more than a half of its initial value.
In the
first column of the second case, in which only acts Bradykinin, the cAMP levels
are a little bit more than 20 micromoles. When the peptide A is added, the
amount of cAMP does not decrease significantly.
The last
case shows that when NGF and Bradykinin are combined together the Adenyl
Cyclase produces a lot of cAMP (almost 80 micromoles), and when the A peptide
is added the production of cAMP decreases abruptly.
The
solution I propose is that NGF has more related with the G protein that
activates Adenyl Cyclase, for that reason it gets more affected when the A
peptide prevents the exchange of GDP for GTP.
The
Bradykinin is not related with the G protein.
NGF is
related with the G protein (couple receptor) and for that reason it gets much affected by the A peptide.
